Organelle Network-Contact
Hyun-Woo Rhee, Ph.D.
Department of Chemistry,
SEOUL NATIONAL UNIVERSITY
Proximity Labeling Tool Development
Although the side chains of peptides and nucleic acids are considered to be inactive at physiological conditions, nature has developed a highly selective method of activating these functional groups to form covalent bonds. Inspired by nature, we will develop new chemical or biochemical methods to create specific covalent bonds to proteins or nucleic acids. This new method of proximity labeling is useful for discovering new biological components that make up the microenvironment in living cells.
In vivo proteomic architecture mapping
So far 18,000 different proteins are known to be expressed in human cells. These proteins are not uniformly distributed throughout the cell, but they are located in highly specific targeted spaces and form microstructures that form a variety of microenvironments. To map localized protein information in a sub-cellular 'space of interest', we will develop a new protein mapping method based on compatible protein label chemistry in living cellular physiology. This new method has the potential to uncover new protein components that are not mapped by traditional protein purification methods such as co-immunoprecipitation. This method can be extended to mapping space-time degraded genomes and metabolites in living cells.
| Contact Information | Websites | |
| rhee@snu.ac.kr |
https://sites.google.com/site/rheeunist/ |
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| Research Interest(s) | ||
| Contact Information |
| rhee@unist.ac.kr |
| Websites |
| https://sites.google.com/site/rheeunist/ |
| Research Interest(s) |